This research aims to study the sterols which is considered, one of the most important minor compounds, and characterizes vegetable oils.Ten items were selected from the planted seeds of safflower in Syria in the spring of 2010. Oil was separated from seed oil extraction with petroleum ether, then the content and content of the sterols were specified by using gas chromatography according to the standard method in the appointment of sterols in olive oil. The study concluded characterize safflower seed oil sterols from their content,