The present study aimed to investigate the capacity of biotin in preventing oxidative stress induced by hydrogen peroxide H2O2 at a concentration of 1% given with consumed drinking water to local male rabbits. 40 male rabbits aged 7-8 months, were used in this study and were divided randomly into four groups, with 10 rabbits within group, as follow: the first group was given standard forage and normal water and considered as control, the second group was given standard forage and 1% H2O2 in water, the third group was given standard forage and 200 mg biotin /kg body weight in water, the fourth group was given standard forage, 1% H2O2 and 200 mg biotin /kg body weight in water. All groups were treated daily for 8 weeks.