Investigate studied the activity of some enzymes in zea maize plant seedlings cells, (Ghota 82) under the influence of phytohormone Auxin & growth regulators 2,4-D and IBA. Maize plant seeds were germinated in water medium for six days in darkness and temperature of 260C. Then stem cells of the developing seedling were incupated in darkness with water or in different solutions of auxin, 2,4-D & IBA (50 mg/l) for 20 houre in 30 0C. Then magared the activity of α-Amylase, RNAase, Catalayse, Guaiacol Peroxidase, Ascorbate oxidase in the stem cells The results showed that the incupation has defferent effects.on hydrolysis and oxidative enzymes in zea maize plant seedlings cells.