This research aims at establishing and testing protocols for isolation, in vitro proliferation, phenotyping, and differentiation of MSCs embedded in umbilical cord tissues of Syrian newborns. MSCs were isolated from two caesarian births depending on their adherence characteristic on plastic surfaces, and cells were cultured in MSC medium for their growth and proliferation. Cell phenotyping was performed by flow cytometry using fluorescent monoclonal antibodies specific to MSCs’ surface markers. Cultured cells were passed several times and a portion of these cells was cryopreserved in liquid nitrogen, and cell viability was assessed. Differentiation of these MSCs into adipocytes was conducted using culture medium Indomethacin and hydrocortisone.