The research Studies genetic relationships among Triticum L. and Aegilops L. species by direct sequencing of PCR-amplified internal transcribed spacer (ITS) of nuclear ribosomal DNA to investigate the polymorphism in nucleotide sequences among 8 Aegilops L. and 7 Triticum L. species. ITS sequences were aligned with CLUSTAL W 2.1 multiple sequence aligment program. The phylogenetic relationships among species were reconstructed using Unweighted Pair Group Mean Arithmetic Average (UPGMA) and neighbor-joining (NJ) methods.