Fusarium oxysporum and F. solani, causal agents of wilt and rot root in citrus seedling are variable pathogens. This study was conducted on the effect of different media in characters, sporulation, and spore germination of fusarium spp. Isolates that were isolated from seed and seedling of citrus. The data revealed that the maximum mycelial growth was obtained in most isolates on Potato Sucrose agar for F. oxysporum and F. solani, followed by Potato Dextrose agar for F. oxysporum, and by Czapek’sDox agar for F. solani. The maximum sporulation was recorded in Potato Sucrose agar medium followed byCzapek’sDox agar . The study also showed that Potato Dextrose agar was the best for germination of spores.