The results of Tissue-blot immunobinding assay (TBIA) of 754 samples of cowpea seeds collected from various regions in Lattakia province has showed the transmission of Pea seed borne mosaic virus (PSbMV) among five virus studied (CMV, BYMV, PSbMV, BBMV and AMV). Pea seed borne mosaic virus was detected on seeds collected from various locations in 24, 64% to 37, and 50%. This result is the first record of seed transmission of PSbMV in cowpea seeds.