Effect of different degrees of temperature, different levels of pH , different periods of light and four media (PDA, OMA, MEA, CzA) was studied in vitro against the growth of C. gloeosporioides causing anthracnose disease of citrus fruits. The results indicated that the growth of fungus was maximum at temperature 30°C and at pH = (5.5,6,6.5), and Light was unnecessary for the growth of C. gloeosporioides. Among the different media tested PDA and OMA supported the maximum growth of fungus.