This study aimed at determining the fish fauna composition in 16th of Tishreen Lake (Alkabeer Alshimali River). A total of 730 individual fishes were collected between April 2012 and March 2013 from five different sites along the lake /Alsafkoon, Wata-Alsheer, Alkerassat, Mazraet Alsheikh Deeb, and Alaqfaas area/. Our results showed the presence of only six main fish species belonging to three families (Tillapia zillii, Cyprinus carpio, Liza abu,Varicorhinus damascinus, Garra rufa and Garra lamta). The results also showed that Tilapia zillii was the most caught fish, and Wata-Alsheer was the richest site in the lake. These results showed a decrease in the number of fish species and their quantities compared to that in previous studies on the lake.