Activity of Ziziphora tenuior L. leaves organic extracts against four pathogenic bacteria, which were isolated from clinical samples and prepared from Al-Assad hospital laboratory in Lattakia, were tested by disc diffusion method through 2014. Results showed that Ziziphora tenuior L. leaves extracts have antibacterial activity against all Gram positive and negative bacteria. Inhibition zones were recorded between 9mm against Pseudomonas aeruginosa and 44mm against Staphylococcus aureus at concentration of 200mg/ml for the methanolic extract. Whereas the inhibition zones for the ethanolic extract were recorded between 7mm at concentration of 40mg/ml against Streptococcus sp. and 34mm at concentration of 200mg/ml against Staphylococcus aureus. These results represent the importance of Ziziphora tenuior L. as a source of natural substances counteraction of pathogenic bacteria.