Isolation and identification of Pseudomonas pachastrellae, Micrococcus brunensis bacteria from soil polluted by petroleum

Two bacterial species were isolated from petroleum hydrocarbon-contaminated soil. The isolates were characterized based on their gram reaction characteristics, morphological and biochemical properties. The results showed two bacteria isolated the first isolated: gram negative short rod, urease and nitrate reduction test showed a positive result , and glucose test showed positive result. And the second isolated: gram positive cocci, gelatin and Citrate utilization test showed a positive result , and maltose, mannitol test showed negative result. They were then incubated at (37) ° C for (48- 72) hours. This is based on comparison of results with Bergey Manual of systematic bacteriology. The isolates were identified to be: Pseudomonas pachastrellae (X1) Micrococcus brunensis (X2).