This research was carried out during the period 08/28/2012 - 07/29/2013 in order to determine the qualitative composition of Puffer Fishes (Tetraodontidae) in the marine water of Latakia. Fish samples were collected from three stations (Borj Islam, Al madina al riyadiah and Jableh), the total of fishes were / 255 / individuals. All fish individuals were classified, preserved and placed at the High Institute of Marine Research Laboratory, some biological factors of the collected fish (K%, GSI%,HIS%) were studied. Results had revealed the presence of five species of Puffer Fish belong to three genera of Tetraodontidae: Lagocephalus sceleratus, L. spadiceus, L. suezensis, Sphoeroides pachygaster, Torquigener flavimaculosus. S. pachygaster species (migratory species from the Atlantic Ocean to the Mediterranean Sea) has been recorded for the first time in the marine water of Syria. Results showed that L. suezensis were more coincidence species in the catch. The amounts caught of Puffer Fishes varied according to the stations; the highest was in Borj Islam. K% was almost similar in all species, while GSI% and HSI% varied according to the species and seasons.