The Mediterranean region is world’s leading olive growing area. A large amount of liquid waste results from olive oil extraction these wastewaters have a different effects an environmental . In this study, four fungal isolates from Samples of soil Polluted by olive mill wastewater ,this isolates follow to several types from deuteromycetes, they are follow to this types : Humicola sp., paecilomyces javanicus, Aspergillus terreus, Aspergillus flavus. They isolated them on tow media (PDA, CzA) and identify them on colony character ,Biometric and compare them with reference studies.