Effect of salinity (Nacl) on seeds germination and seedling growth cultured in laboratory of two cultivars of soybean [Glycine max (L) Merr.]


Abstract in English

The objective of this study is to compare the effect of five different Nacl concentration (0,50,100,150,200 mM) on seeds germination and seedling growth of two cultivars of soybean (sb-44,sb-172). Seeds were germinated in petri dishes with sterile filter paper moistened with 10 ml of test solution at 25 c˚ in dark . The obtained results showed that germination percentage , length of root and hypocotyl were decreased with the increase of the Nacl concentration in the medium. The best values was from cultivar sb-44 . After seven days of incubation , significant differences were observed between control and treated seeds . Germinated seeds were subcultured on basal Murashige and Skoog medium solid at the same Nacl concentration . The roots growth was mor affected by 200 mM Nacl compared to seedling growth . Plantlets were transfered into pots with nutrient soil , irrigated with saline water and were adapted at laboratory . we obtained good plants grew to maturity in 10- 12 weeks .

References used

ALMANSO URI,M.; KINET,J.M and LUTT,S. Effect of salt and osmotic stresses on germination in durum wheat. Plant soil, 231, 2001, 243-254
AMIRJANI,M.R. Effect of salinity stress on growth, mineral composition, proline content, antioxidant enzymes of soybean. Amer.J. of plant physiology. 5,2010, 350- 360
ATAK,M..; KAYA, N.D.; CIKILI,Y. and CIFTCI, C.Y. Effect of Nacl on the germination, seedling growth and water uptake of triticale. Turk.J.Agric.for.30,2006,39-47

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