The aim behind this study was to isolate and characterize fungi from fermented olive mill wastewater to determine the superior one that can resist high organic load and total phenol of fresh Olive Mill Wastewater (OMW). Two different media Czapak Dox Agar (CzA) and Potato Dextrose Agar (PDA) were used for identification of fungi by studying macroscopic and microscopic characteristics. However, Aspergilluss sclerotiorum, Paecilomyces sniveus and Mucor nircinelloides had had been identified . After testing the isolates on fresh olive mill wastewater solid mediums OMWA1 (50%OMW), OMWA2 (75%OMW) and OMWA3 (100%OMW). Whereas P. niveus proves that was the superior one, which can resist high concentration of fresh olive mill wastewater.