The free iodine (I2) in povidone-iodine (PVP-I) solutions is responsible for its bactericidal activity, the bactericidal effect is steadily increased with the free iodine concentration. This study aims to compare some of povidone-iodine solutions common at the local market with one solution produced by Mundipharma company (Betadine®) which used as a reference. Commercial samples (PVP-I 10%) were obtained from three local companies (A, B, C), with one batch from each company. The research included controlling free iodine concentration, pH and evaluation of bactericidal activity by using disc diffusion method and rapidity of the bactericidal activity on both Staphylococcus aureus and Escherichia coli. The local samples solutions showed low pH values compared with Betadine®, furthermore, all the solutions extracted by heptane showed high levels of free iodine with a preference for one local company. The high level of free iodine was accompanied with an increase of inhibition diameter and rapidity of bactericidal effect.