Influence of Some Home Preparation and Storage Conditions on Ascorbic Acid Content of Infant Formula

Vitamin C concentration was studied in samples of infant formula from 5 companies (referenced A-E) using the 2,6-Dichlorophenolindophenol titration (AOAC, 2006) method. Results revealed that ascorbic acid content in formulas D and C was the closest to its recommended daily intake (RDI) and to the concentrations which are mentioned on the label of the samples. Storage conditions at room temperature or in the refrigerator did not affect ascorbic acid levels. In comparison to vitamin C concentrations in milk dissolved in tap water, its levels decreased when water was warm (40°C) or hot (100°C), and its concentrations further declined in dissolved form kept for two hours after preparation.