Gestational Diabetes Mellitus (GDM) is one of the most common complications during pregnancy. The prevalence of gestational diabetes (GDM) is increasing all over the world. Hence, the impact of GDM on maternal and infant health is an important topic of research. Objective: The aim of the study was to determine the prevalence of GDM, and to assess the impact of universal screening to detect Gestational diabetes mellitus. Subjects and methods: A representative sample of 760 pregnant women who attended the antenatal department of Al Assad Hospital in Lattakia were surveyed during the period from June 1st, 2013 to December 1st, 2014. The questionnaire covered variables related maternal age, gestational age, BMI, and women underwent the diagnostic tests for GDM. 73 pregnant women had positive results.