This research was conducted to apply the technique of Artificial Insemination in poultry for the first time in the Syrian Arab Republic , and study the differences between the impact of the use of fresh or stored semen on the fertilization rate in hatching eggs . Results showed that the response to massage was different between cocks, It was greater in the cock A and cock B while the cock C was the least response to massage. The greater volume of ejaculate was obtained from the cock A( 0.072) ml ,and the least volume of ejaculate was obtained from the cock B (0.05) ml . The volume of ejaculate obtained from the three cocks was ranged between (0.049 -0.072) ml. The value of X in the count ranged between (230 - 237) sperm, semen concentration ranged between (4.6 - 4.7) billion sperm / ml. It also found that Insemination with a fresh semen injection yielded fertilization rate (91%), While insemination with semen injection cooling at the heat +4 ̊ C for 48 hours yielded fertilization rate (67%) .