The concentration of ethanol in blood and urine provides important evidence in criminal and civil litigation when alcohol- related crimes are investigated (e.g., drunk driving )Therefore. Ethanol analysis is the most frequently performed assay in forensic toxicology laboratories . .However, the risk for artifactual formation of ethanol, especially in postmortem specimens, always needs to be considered . It is often difficult to distinguish between postmortem ethanol production and antemortem alcohol ingestion . Ethanol synthesis could be produced by many species of bacteria or yeasts ,which is also influenced as storage temperature, and the interval between death and autopsy increase .