Chemotherapy drugs cannot tell the difference between of normal cells and cancer cells. This means normal cells are damaged and this results in side effects, This damage does not stop in the patient who receiving treatment but also extends to all employees in the health care sitting. Nurses are the main groups that are exposed to these drugs in hospital setting Generally, the occupational activities that pose to greatest risk of exposure are the preparation ,Transporting cytotoxic drugs, administration of antineoplastic agents, cleaning of chemotherapy spills, and handling of waste. Nurses require specialized knowledge and skills in order to ensure safety for both patients life and for their own safety of the jobs. Because of available a lot of chemotherapy guidelines we Must assess knowledge and skills nursing staff and assess the extent of their commitment to these guidelines. Objective: The objectives of this study were to assessment of knowledge and skill of oncology nurses in chemotherapy administration.