The objective of this study was isolate and identify bacteria which was carried mechanically and biologically on house fly (Musca domestica )in poultry and animal farms .in this study samples of M. domestica were collected from 6 farms of animal farms in different areas of Latakia . Bacteriological laboratory tests were conducted for (80) samples of M. domestica and (10) samples of dead bird . bacterial cultures on selective or general environments showed different strains of micrococcus (Staphylococcus spp. ,Enterococcus spp.) and Bacillus (Pasteurella spp. , E.coli , Salmonella spp. , Pseudomonas spp.) these species were further classified using the API kit system . API tests was showed entrant isolations between house fly and ill birds which important in disease locality : Salmonella choleraes, Staphylococcus aureus, E.coli, klebsiella pneumonia.