This study was done in special apiary (Latakia province) to define the effect of adding some vitamins on the nutrition of the local honeybee colonies at the rate of acceptation in the plastic and wax cups are grafted with larva; and in the productivity of royal jelly. The results showed that best of acceptation rate was achieved by vitamin C treatment that arrived to 85.41% in the wax cups and 75.83% in the plastic cups. According to results of the statistical analysis, it significantly surpassed upon all other treatments, whereas there wasn’t any significant difference between the treatment with vitamin Aand control, no matter what kind of cups it used. The results of average weight of the royal jelly in the cup showed that the treatment of vitamin C achieved the best value that reached to 0.348 g of the wax cups and 0.312 g of plastic cups.The statistical analysis showed superiority of the treatment vitamin C and B upon other treatments (vitamin A and control) while using the wax cups, whereas there was no significant difference in the average weight of the royal jelly between the two kinds of cups when treating with vitamin A and control.