A survey of Tomato spotted wilt virus (TSWV, Tospovirus, Bunyaviridae) on some solanaceous crops and associated reservoir weeds was conducted between March and November 2013. 983 field samples was collected (703 Solanaceous crops, 280 associated weeds), and 218 greenhouse samples (190 Solanaceous crops, 28 associated weeds). Tissue Blot Immunobinding Assay TBIA were performed to detect TSWV. Results of TBIA of tested samples showed the natural infection of TSWV in Lattakia by 22,06%. The percentage infection in field crops was 8,82% on solanaceous crops and 62,5% on associated weeds and It was in greenhouse crops 2,11% on solanaceous crops and 85,71% on associated weeds. This is the first report in Syria of Tomato spotted wilt virus on Tobacco, Eggplant, and several weeds.