Experimentally detection of genes coding for PSA surface proteins in the genome of Leishmania major strain locally isolated
published by Damascus University
in 2013
in
and research's language is
العربية
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Abstract in English
That study was based on a BLAST search analysis performed against the
complete genome sequences using as a query sequence some of the PSA protein
sequences reported in the literature. In our study, we have first isolated,
cultivated, typed and strain of L. major. Then we have investigated
experimentally the complete list of PSA genes coding for membrane proteins in
its genome, using PCR approach based on specific primers. In conclusion, we
have detected the physical presence of 22 different genes coding for membrane
proteins in the genome of Syrian strain of L. major.
References used
Alexander, J. and D. G. Russell (1992). "The interaction of Leishmania species with macrophages." Adv. Parasitol 31: 175-254
Beetham, J. K., J. E. Donelson, et al. (2003). "Surface glycoprotein PSA (GP46) expression during short- and long-term culture of Leishmania chagasi." Mol. Biochem. Parasitol. 131(2): 109-117
Ben Achour, Y., M. Chenik, et al. (2002). "Identification of a disulfide isomerase protein of Leishmania major as a putative virulence factor." Infect. Immun. 70(7): 3576-3585