Construction of pRMES and pTMES plasmids to test their expression ability of Nanobodies via the efficient T7 promoter
published by Damascus University
in 2013
in
and research's language is
العربية
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Abstract in English
Nanobody technology is considered as a promising molecular biology
technique performed by means of the genetic engineering of special type of
antibodies, existing exclusively in Camelidea. It enables the obtaining of small
proteins, referred to as Nanobodies, which are characterized by high stability
and solubility, are able to link to their specific antigens. After production, the
Nanobody genes are cloned within plasmids of protein expression in bacteria,
allowing their stable and continuous production for research and applied
purposes.
References used
Abbady, A. Q., Al-Daoude, A., Al-Mariri, A., Zarkawi, M., and Muyldermans, S. (2012). Chaperonin GroEL a Brucella immunodominant antigen identified using Nanobody and MALDI-TOF-MS technologies. Vet. Immunol. Immunopathol. 146, 254-263
Abbady, A. Q., Al-Mariri, A., Zarkawi, M., Al-Assad, A., and Muyldermans, S. (2011). Evaluation of a nanobody phage display library constructed from a Brucellaimmunised camel. Vet. Immunol. Immunopathol. 142, 49-56
Abo Assali, L., Al-Mariri, A., Hamad, E., and Abbady, A.Q. (2012). Immunodetection of the recombinant GroEL by the Nanobody NbBruc02. World J. Microbiol. Biotechnol. 28, 2987-2995