Optimization of Cellulase Enzyme Production Conditions from Bacillus cereus 21 Using Submerged Fermentation


Abstract in English

The conditions for producing cellulase enzyme were optimized using corn husks as substrate in submerged fermentation. The effects of five parameters (incubation temperature, pH, substrate concentration, inoculum volume, fermentation time) on the strain growth and enzyme production were studied. For this purpose the statistical program Minitab and the statistical design Response Surface Methodology (RSM) were applied.

References used

Abou-Taleb, K. A., W. A. Mashhoor, A. Sohair, M.S. Sharaf, and H. M. Hoda. 2009. Nutritional and Environmental Factors Affecting Cellulase Production by Two Strains of Cellulolytic Bacilli, Australian Journal of Basic and Applied Sciences 3(3): 2429-2436
Bailey, M. J. and K. M. H. Nevalainen. 1981. Induction, isolation and testing of stable Trichoderma reesei mutants, Enzyme Microb. Technology 3: 153-157
Buchert J., Suurnakki A., Tenkanen M. and Viikari L. 1996. Enzymatic characterization of pulps, in enzymes for pulp and paper processing, Edited by T. W. Jeffries, L Viikari, ACS Symp Ser 655. 38-43

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