This research deals with amain steps related to preparing live food which is necessary to grow up larvae of different species of marine family Penaeidae prawn, in this study the isolation of two species of phytoplankton one cells they are Chlorella sp, Tetraselmis sp, and the specimens are collected from littoral of jableh and rearing them up in hard and liquid medium in laboratory in Faculty of Sciences, Tishreen University, in the period between April and august 2013.