In this search we characterized 8 double purposes olive varieties (Mawi Istanbuli, Khilkhali Khishen, Dan, Mniekri, Doaibli, Khilkhali Saghir, Karamani Modabal, Sourani), which cultivated in ACSAD Gene bank in Gellin by ISSR molecular markers to determine the relationship between these varieties, we noticed that these varieties are so close to each others depending on quantitive traits, which ranged from 60% and 85%, it was noticed that Mneikri was apart from the other varieties in its traits, polymorphic rate was 92.94%.