This study discusses in-duct UVGI efficiency to disinfect O.R and ICU supplied air. Experiment setup was designed using three ultraviolet lamps were placed at the upper side of experimental duct, equipped with variable speed turbine to simulate flow rates of ICU and O.R. In order to determine organism irradiance time, UV intensity distribution is measured at different levels in duct, Thenthese parameters combined in simulation program executed under LABVIEW programming environment to determine in-duct disinfection efficiency in relation to ICU and O.R designing requirement and sterility level.