Twelve growing Shami heifers available in Dier-Al-Hajar Station for improving Shami Cattle weighing 115±3 kg and 178±4 days old were used to determine the weekly hormonal concentrations of progesterone (P4) and estradiol 17-β (E2) during the pre- and puberty stages (6-14 mos). Blood samples were collected weekly from the Jugular vein using vacutainer tubes supported with K3EDTA.