This research aims to study the effects of some oxidoreductase enzymes activity in the seedlings of maize plant stem cells, which Cultured in the present of the following cations: Na+, K+, Mg2+, and Ca2+. (GHOTA 82) yallow corn seeds were used, and cultured for six days in water and in solution of cations with different concentrations. In brief, preparation and titration processes consist of extraction of enzymatic solutions from plant stem cells seedlings by buffer solutions (at appropriate PH for each enzyme). The results show that the activity of used enzymes increases with the increasing concentrations of the used cations from 1 to 6 g/l by comparison with those of cultured samples in water. Besides the difference in the effect of cations was limited by the concentrations range between 6-9 g/ l.