Ziziphora canescens is a species of important medicinal plants in Syria due to its medicinal properties as antibiotic, flavors and spices in various foods. This plant is important, especially in folk medicine in some areas (Kalamoon) on the one hand, and retreat of its spread which may lead eventually to its extinction on the other hand, so a protocol for rapid micropropagation has been developing by using lateral and apical buds on nutrient media MS supplemented with different types and concentrations of plant growth regulators.