International demand of energy resources increase as result of economical and social accelerated development, and under scenarios which predicted depletion of fossil fuel resource, the efforts trends to alternative energy resource as biofuel, where a native strain of Botryococcus braunii was isolated from Room reservoir in Sweida-Syria and used to produce biofuel, production conditions optimized in flasks, then turn to work on photobioreactor which made by native experience. Dry biomass, lipid content of Botryococcus braunii and optical density were calculated to express algal growth.