This study were conducted on 450 samples of raw chicken meat ( 150 samples of thighs- 150 samples of breasts -150 samples of wings ) were collected from retail market in Hama city, to detect contamination of staphylococcus. Bacteriological and biochemical tests results showed 407 samples positive for staphylococcus . contained S. aureus 73 (16.2% ) isolated and coagulase-negative Staphylococcus 334 (83.8%) isolated out of total samples. The results of Multiplex PCR test conducted on S. aureus isolates, showed that 37(50.7%) isolates harbored at least one enterotoxin gene, with sea being the most frequently encountered ones.