Prevalence of Hepatitis B and C infections was assessed among individuals undergoing Pre-marital Tests in Pre-marital Clinic & Laboratory in Homs city – Syria. Enzyme immunoassay technique was used for detection of hepatitis B surface antigen (HBs-Ag) and antibodies to hepatitis C virus (Anti-HCV). Among 20290 subjects, 288 (1.4%) were positive for HBs-Ag, and 24 (0.12%) were positive for Anti-HCV. Prevalence of Hepatitis B and C was more common in male subjects (84.4% for Hepatitis B and 75% for Hepatitis C) in comparison to female (15.6% for Hepatitis B and 25% for Hepatitis C).