The Effect of Temperature Factor on the Detection of virus Caprine Arthritis- Encephalitis Virus (CAEV), using the technique of Real Time-PCR


Abstract in English

This study has been conducted to adapt a quantitative RT-QPCR to detect the least amount of virus and tissue culture pathogenic dose (TCID50) of CAEV showed cytopathogenic effect (CPE) from clinical samples.

References used

Fieni F., Pellerin J.L., Roux C., Poulin N., Baril G., Fatet A., Valasg S., Chatagnon G., Mermillod P., Guignot F. (2012) Can caprine arthritis encephalitis virus (CAEV) be transmitted by in vitro fertilization with experimentally infected sperm? Theriogenology 77. 644–651
Cheevers WP, Knowles DP, McGuire TC, Cunningham DR, Adams DS, Gorham JR. Chronic disease in goats orally infected with two isolates of the caprine arthritis-encephalitis lentivirus. Lab Invest 1988;58:510–7
Chebloune Y, Sheffer D, Karr BM, Stephens E, Narayan O. Restrictive type of replication of Ovine/caprine lentiviruses in ovine fibroblast cell cultures. Virology 1996;222:21–30

Download