The aim of this study is isolating brucella and using polymerase chain reaction technique for the molecular characterization of Brucellamelitensis,which causes caprine abortion. It also aimsto determine the rate of caprine abortion that is caused by Brucellamelitensisin the Middle region of Syria. For this purpose 58 abortion samples were collected (36 aborted fetuses, 22 vaginal swabs). The results of bacterial isolation and polymerase chain reaction reveal that the prevalence of caprine abortion caused by Brucella melitensis was 53.4%. The study also reveals that using stomach contents from caprine aborted fetus can be the best site for brucella isolation. Running differentiation tests to distinguish field strains from vaccinal strains refer to the presence of 2 isolates(6.4%) belonging to vaccinal strains(Rev1) from 31 isolates of Brucella melitensis.