Content control of some commercial products containing amino acid available in the local market


Abstract in English

The aim of this study was to control the content of some commercial products containing amino acid (Arginine) available in the local market in addition to other products containing creatine and protein. Three methods were used for quantitative determination of amino acids Kjeldahl method depending on the total content of nitrogen, assay by the visible radiation using Spectrophotometer after ninhydrin derivatization and high performance liquid chromatography with pre-column O-phtadelaldehyde derivatization using fluorescence detector. There were no significant differences in the results obtained by the three used methods. The results revealed that dietary supplements containing creatine and protein were conform to the European pharmacopoeia specifications, regarding products containing arginine used as medication and locally manufactured, they were conform to pharmacopoeia specifications while the illegal dietary supplement containing arginine were not conform.

References used

AOAC International, Official Methods of Analysis, 17th ed., AOAC International, Arlington, VA, 2000
BELTIZ. H.D.; GROSCH. W.; SCHIEBERLE. P. Food Chemistry. 4th revised and extended . Springer, Germany, 2009, 8-89
BEMBEN. MG.; LAMONT. HS. Creatine supplementation and exercise performance: recent findings. Sports Med, (35) 2005 , 107-25
British Pharmacopoeia Volume III , 2012
European pharmacopoeia, the Council of Europe, 2005

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