A newly developed methodology based on image analysis technique has been used to measure fiber openness and the results are compared with those of the traditional method of measurement of fiber openness. The role of different opening process parameters in deciding the openness of fibers obtained by image analysis technique is found to be much higher than that obtained by beaker test method. It is found that the higher speed of opening rollers and higher angle of grid bars improve fiber openness appreciably, but with little deterioration in fiber quality. The higher fiber openness ultimately results into more regular and less imperfect yarn. With the increase in fiber openness, yarn strength and yarn breaking elongation increase initially and then decrease. Yarn hairiness almost unchanged with the increase in fiber openness.