purification of Carotenoids from Mutant Yeast R.mucilaginosa(A23-M) Identified Using High Performance Liquid Chromatography HPLC

Carotenoids were separated from the wild type yeast R.mucilaginosa (A23) and its UV mutant at (254 nm) R.mucilaginosa (A23-M) using thin layer chromatography (TLC).The results showed the wild type yeast gave three color patches, β-Carotene,Torulene and Torularhodin with Rf values of 0.9, 0.7 and 0.2 respectively, while the mutant yeast gave only one spot of color ofTorularhodin at thethe value of Rf = 0.2. Carotenoid produced by mutant yeast R.mucilaginosa (A23-M) was purified using a column stocked with polychlorinated Hyflo Super Cel and magnesium oxide with a ratio of (1: 2). The purified carotenoid was analyzed by high performance liquid technology chromatography HPLC at a wavelength 495 nm showing that there was only one colored compound which was Torularhodin