This study aims to evaluat of the effect antibacterial for some materials which are used dressing and irrigating fluids in infected root canal then compare of the effectiveness of each of The irrigating fluids and materials used Dressing in infected root canal The sample of study was 90 cases a single infection root canal , were divided into three group and applied to the total of each such substance(calcium hydroxide with sodium hypochlorite ,calcium hydroxide with Iodoform, chx) and then conducted the transplant bacterial. The cases were evaluated in terms of results of bacterial culture. The study showed that chx best antidote infection of calcium hydroxide with sodium hypochlorite and calcium hydroxide with Iodoform