It was collected 2990 blood samples from various ages of chicken of broiler, layer and breeder flocks distributed from different regions of SAR, in order to detect Mycoplasma Galisepticum that used in Rapid sero- agglutination test and indirect ELISA test. The study reported seroprevalence of positive cases using rapid sero- agglutination test 33% in broiler breeder, 1% in broiler chicken and 43% in layer chicken and overall prevalence 24%. And by using ELISA test, the reported seroprevalence values 27% in breeder, 8% in broiler and 33% in layer chicken and overall-prevalence 19%.