Species of the genus Campylobacterare recognized as the main cause of foodborne disease in both developing and developed countries. They are main causative of diarrhea and gastroenteritis worldwide. Staphylococcus bacteria especially S. aureus are responsible of food poisoning due to their ability to produce enterotoxins. Traditional methods which are used to identify bacteria are time-consuming and labor intensive and very expensive. Thirteen isolates of Campylobacter and twenty-two isolates of Staphylococcus were isolated from a variety of foods. Traditional methods based on biochemical tests were used for identification in addition to laser light-scattering technique to discriminate isolates. Laser light-scattering technique showed the ability to distinguish all isolate in high accuracy, rapid and low costs manner, thus it may represent a potential alternative to traditional methods to identify these bacteria. So it is a very important issue to find new alternative methods characterized by high accuracy, low costs and rapidity in results achievement, to replace traditional methods, thus laser light-scattering may be a possible alternative