The period of a newborn is a critical one. This study aims to assess the quality of the performance of the nursing staff in the provision of nursing care for a newborn in the incubator. This is an important and critical procedure for newborns in the Neonatal Intensive Care Unit NICU. The study included 30 nurses from the nursing staff in the Department of incubators at three hospitals. A questionnaire has been developed consisting of two parts: The first part (I) has questions related to the demographic information of research sample. The second part (II): A checklist was developed by the researcher based on global standards related to some private nursing procedures provided for newborns within the incubator. These measures are: -Washing hands -Clean the incubator -Venous catheter - Taking vital signs ( temperature - Breathe - Pulse ) -Infection control within the incubators -Oxygen therapy – Phototherapy. Findings show a third of the sample had a weak performance in all nursing procedures, while the remaining third (33%) had an average level of performance. The present study showed that there is no important significant relationship between the level of performance in the nursing department incubators and both (qualification and years of experience in the nursing department incubators). The study also revealed the presence of an important significant relationship between the level of performance in the nursing department incubators and both (age and years of experience of nursing staff) for the total nursing procedures