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Simulation and Experimental Investigation of Cellular Material Breakage Using the Pulsed Electric Field Treatment

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 Added by NLebovka
 Publication date 1999
  fields Physics
and research's language is English




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We consider the simplified dielectric breakage model used for simulation of the kinetics of cellular material breakage under the pulsed electric field (PEF) treatment. The model is based on an effective media approximation, which includes equations with the same morphology parameters as in percolation theory. The probability of a whole cell breakage by the pulse with $t_{i}$ duration is estimated on the basis of electroporation theory. We account for the bridging effect resulting from the deviations of the local conductivity near the selected cell from the average effective media conductivity. The most important feature of the proposed model is the existence of the ``jamming behaviour occurring sometimes in experimental observations of the biological tissue breakage. The different transitions corresponding to the ``jamming steps are identified. The experimental results are obtained for thin apple slices treated with electric pulses at field strengths $E=0.2-2.2$ kV cm$^{-1}$, pulse durations $t_{i}=10-100$ $mu$s, pulse repetition times $t=10-100$ ms and the number of pulses $N=1-100000$. The model gives results consistent in general with the experimental observations. We discuss the correlation between the degree of cellular material destruction, field strength, time of PEF treatment and power consumption.



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We present results of numerical and experimental investigation of the electric breakage of a cellular material in pulsed electric fields (PEF). The numerical model simulates the conductive properties of a cellular material by a two-dimensional array of biological cells. The application of an external field in the form of the idealised square pulse sequence with a pulse duration $t_{i}$, and a pulse repetition time $Delta t$ is assumed. The simulation model includes the known mechanisms of temporal and spatial evolution of the conductive properties of different microstructural elements in a tissue. The kinetics of breakage at different values of electric field strength $E$, $t_{i}$ and $Delta t$ was studied in experimental investigation. We propose the hypothesis for the nature of tissue properties evolution after PEF treatment and consider this phenomena as a correlated percolation, which is governed by two key processes: resealing of cells and moisture transfer processes inside the cellular structure. The breakage kinetics was shown to be very sensitive to the repetition times $Delta t$ of the PEF treatment. We observed correlated percolation patterns in a case when $Delta t$ exceeds the characteristic time of the processes of moisture transfer and random percolation patterns in other cases. The long-term mode of the pulse repetition times in PEF treatment allows us to visualize experimentally the macroscopic percolation channels in the sample.
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