We investigate the Initial Mass Function and mass segregation in super star cluster M82-F with high resolution Keck/NIRSPEC echelle spectroscopy. Cross-correlation with template supergiant spectra provides the velocity dispersion of the cluster, enabling measurement of the kinematic (virial) mass of the cluster when combined with sizes from NICMOS and ACS images. We find a mass of 6.6 +/- 0.9 x 10^5 M_sun based on near-IR light and 7.0 +/- 1.2 x 10^5 M_sun based on optical light. Using PSF-fitting photometry, we derive the clusters light-to-mass ratio in both near-IR and optical light, and compare to population synthesis models. The ratios are inconsistent with a normal stellar initial mass function for the adopted age of 40 to 60 Myr, suggesting a deficiency of low-mass stars within the volume sampled. King model light profile fits to new HST/ACS images of M82-F, in combination with fits to archival near-IR images, indicate mass segregation in the cluster. As a result, the virial mass represents a lower limit on the mass of the cluster.