We present time-resolved optical spectroscopy and photometry, and far-ultraviolet spectroscopy of HS 0728+6738, a cataclysmic variable discovered in the Hamburg Quasar Survey. We show that the system is a new eclipsing member of the SW Sex class of CVs with an orbital period of 3.21 hours. We derive an orbital inclination of ~85 +- 4 degrees from the average eclipse profile, making HS 0728+6738 the highest inclination SW Sex star known. The optical and far-ultraviolet emission lines are not or only weakly occulted during the eclipse, indicating the presence of line-emission sites either far outside the Roche lobe of the primary or, more likely, above the orbital plane of the binary. The photometric light curves exhibit fast variability with a period of ~7 min, which might be related to the spin of the white dwarf.
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