We present photometric and spectroscopic analyses of gravity (g-mode) long-period pulsating hot subdwarf B (sdB) stars. We perform a detailed asteroseismic and spectroscopic analysis of five pulsating sdB stars observed with {it TESS} aiming at the global comparison of the observations with the model predictions based on our stellar evolution computations coupled with the adiabatic pulsation computations. We apply standard seismic tools for mode identification, including asymptotic period spacings and rotational frequency multiplets. We calculate the mean period spacing for $l = 1$ and $l = 2$ modes and estimate the errors by means of a statistical resampling analysis. For all stars, atmospheric parameters were derived by fitting synthetic spectra to the newly obtained low-resolution spectra. We have computed stellar evolution models using {tt LPCODE} stellar evolution code, and computed $l = 1$ g-mode frequencies with the adiabatic non-radial pulsation code {tt LP-PUL}. Derived observational mean period spacings are then compared to the mean period spacings from detailed stellar evolution computations coupled with the adiabatic pulsation computations of g-modes. The atmospheric parameters derived from spectroscopic data are typical of long-period pulsating sdB stars with the effective temperature ranging from 23,700,K to 27,600,K and surface gravity spanning from 5.3,dex to 5.5,dex. In agreement with the expectations from theoretical arguments and previous asteroseismological works, we find that the mean period spacings obtained for models with small convective cores, as predicted by a pure Schwarzschild criterion, are incompatible with the observations. We find that models with a standard/modest convective boundary mixing at the boundary of the convective core are in better agreement with the observed mean period spacings and are therefore more realistic.
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