The uncertainty relation lies at the heart of quantum theory and behaves as a non-classical constraint on the indeterminacies of incompatible observables in a system. In the literature, many experiments have been devoted to the test of the uncertainty relations which mainly focus on the pure states. Here we present an experimental investigation on the optimal majorization uncertainty for mixed states by means of the coherent light. The polarization states with adjustable mixedness are prepared by the combination of two coherent beams, and we test the majorization uncertainty relation for three incompatible observables using the prepared mixed states. The experimental results show that the direct sum majorization uncertainty relations are tight and optimal for general mixed systems.
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